Part:BBa_K1484317:Experience

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Applications of BBa_K1484317

This part converted p-coumaric acid into 4-hydroxybenzalacetone, which is also referred to simply as benzalacetone and is a precursor to raspberry ketone.

User Reviews

When we used it in Cambridge, England, it worked really well!

E. coli expressing this part were incubated for two days in M9 minimal medium. We obtained the following spectrum after shaking the culture with ethyl acetate and running on an LCMS setup:

The peak marked with a green star, when passed through a mass spectrometer, gave peaks characteristic of benzalacetone. The large peak to its left is p-coumaric acid, so conversion was incomplete but significant.

Beware that when E. coli were transformed to also express 'benzalacetone reductase' (BBa_M36705), raspberry ketone was NOT produced. This agrees with the findings of the lab that this gene was cloned by, led by Dr Jules Beekwilder in Wageningen, The Netherlands, that this part BBa_M36705 was not shown to exhibit benzalacetone reductase activity either in vivo or in vitro.

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